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KMID : 0811720120160020107
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Korean Journal of Physiology & Pharmacology
2012 Volume.16 No. 2 p.107 ~ p.112
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3,4,5-Trihydroxycinnamic Acid Inhibits LPS-Induced iNOS Expression by Suppressing NF-?B Activation in BV2 Microglial Cells
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Lee Jae-Won
Bae Chang-Jun
Choi Yong-Jun
Kim Song-In
Kim Nam-Ho
Lee Hee-Jae
Kim Sung-Soo
Kwon Yong-Soo
Chun Wan-Joo
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Abstract
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Although various derivatives of caffeic acid have been reported to possess a wide variety of biological activities such as neuronal protection against excitotoxicity and anti-inflammatory property, the biological activity of 3,4,5-trihydroxycinnamic acid (THC), a derivative of hydroxycinnamic acids, has not been clearly examined. The objective of the present study is to evaluate the anti-inflammatory effects of THC on lipopolysaccharide (LPS)-stimulated BV2 microglial cells. THC significantly suppressed LPS-induced excessive production of nitric oxide (NO) and expression of iNOS, which is responsible for the production of iNOS. THC also suppressed LPS-induced overproduction of pro-inflammatory cytokines such as IL-1?and TNF-?in BV2 microgilal cells. Furthermore, THC significantly suppressed LPS-induced degradation of I?B, which retains NF-?B in the cytoplasm. Therefore, THC attenuated nuclear translocation of NF-?B, a major pro-inflammatory transcription factor. Taken together, the present study for the first time demonstrates that THC exhibits anti- inflammatory activity through the suppression of NF-?B transcriptional activation in LPS-stimulated BV2 microglial cells.
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KEYWORD
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3,4,5-Trihydroxycinnamic acid (THC), BV2 microglial cells, Lipopolysaccharide, iNOS, NF-?B
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